dna clean & concentrator-5 (Zymo Research)
Structured Review
Dna Clean & Concentrator 5, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 8450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/concentrator+5/DNA+Clean+%26+Concentrator-5/custom%40d4014%4042667331
Average 99 stars, based on 8450 article reviews
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other:Article Title: De novo biosynthesis of halogenated benzoxazinoids in Nicotiana benthamiana. Article Snippet: Platinum SuperFi II PCR Master Mix (Thermo Fisher Scientific) was used for gene amplification according to manufacturer's instructions. Purification:Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures. Article Snippet: .. The 10-20 μL of PBS was purified on Zymo RNA Clean and Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures. Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and Article Title: Loss of host factor-mediated m 6 Am methylation of the viral RNA cap impairs SARS CoV-2 replication Article Snippet: The decapped RNA was ligated with an RNA adapter in a total 25 μL reaction (RNA 9.25 μL, 10x T4 RNA ligase buffer 2.50 μL, 5 μM 5′ Adapter in 3.0 μL (15 pmol total), RNasin plus 1.0 μL (40units), T4 RNA ligase 2.0 μL (NEB, M0204S), 50% PEG8000 6.25 μL, 25 mM ATP 1.0 μL) overnight at 16°C. .. The next day, RNA was purified with RNA Clean and Polymerase Chain Reaction:Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures. Article Snippet: .. The 10-20 μL of PBS was purified on Zymo RNA Clean and Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures. Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and Construct:Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures. Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and Nick Translation:Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures. Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and Ethanol Precipitation:Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures. Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and cDNA Synthesis:Article Title: Loss of host factor-mediated m 6 Am methylation of the viral RNA cap impairs SARS CoV-2 replication Article Snippet: The decapped RNA was ligated with an RNA adapter in a total 25 μL reaction (RNA 9.25 μL, 10x T4 RNA ligase buffer 2.50 μL, 5 μM 5′ Adapter in 3.0 μL (15 pmol total), RNasin plus 1.0 μL (40units), T4 RNA ligase 2.0 μL (NEB, M0204S), 50% PEG8000 6.25 μL, 25 mM ATP 1.0 μL) overnight at 16°C. .. The next day, RNA was purified with RNA Clean and Binding Assay:Article Title: Loss of host factor-mediated m 6 Am methylation of the viral RNA cap impairs SARS CoV-2 replication Article Snippet: The decapped RNA was ligated with an RNA adapter in a total 25 μL reaction (RNA 9.25 μL, 10x T4 RNA ligase buffer 2.50 μL, 5 μM 5′ Adapter in 3.0 μL (15 pmol total), RNasin plus 1.0 μL (40units), T4 RNA ligase 2.0 μL (NEB, M0204S), 50% PEG8000 6.25 μL, 25 mM ATP 1.0 μL) overnight at 16°C. .. The next day, RNA was purified with RNA Clean and |
