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dna clean & concentrator-5  (Zymo Research)


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    Structured Review

    Zymo Research dna clean & concentrator-5
    Dna Clean & Concentrator 5, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 8450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/concentrator+5/DNA+Clean+%26+Concentrator-5/custom%40d4014%4042667331
    Average 99 stars, based on 8450 article reviews
    dna clean & concentrator-5 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: De novo biosynthesis of halogenated benzoxazinoids in Nicotiana benthamiana.
    Article Snippet: Platinum SuperFi II PCR Master Mix (Thermo Fisher Scientific) was used for gene amplification according to manufacturer's instructions.

    Purification:

    Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures.
    Article Snippet: .. The 10-20 μL of PBS was purified on Zymo RNA Clean and Concentrator-5 (catalog number R1015) to remove PCR inhibitors. ..

    Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures.
    Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and Concentrator-5 (catalog number R1015), not by ethanol precipitation. ..

    Article Title: Loss of host factor-mediated m 6 Am methylation of the viral RNA cap impairs SARS CoV-2 replication
    Article Snippet: The decapped RNA was ligated with an RNA adapter in a total 25 μL reaction (RNA 9.25 μL, 10x T4 RNA ligase buffer 2.50 μL, 5 μM 5′ Adapter in 3.0 μL (15 pmol total), RNasin plus 1.0 μL (40units), T4 RNA ligase 2.0 μL (NEB, M0204S), 50% PEG8000 6.25 μL, 25 mM ATP 1.0 μL) overnight at 16°C. .. The next day, RNA was purified with RNA Clean and Concentrator-5 (Zymo research, R1016) following the long RNA purification protocol and subjected to first-strand cDNA synthesis using 0.5 μg (1 μL, 50 μM stock) primer pWhSF-ORF1a-R18-655 ( ) binding to the ORF1a, and superscript RTII (ThermoFisher Scientific, Cat. 18064022). ..

    Polymerase Chain Reaction:

    Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures.
    Article Snippet: .. The 10-20 μL of PBS was purified on Zymo RNA Clean and Concentrator-5 (catalog number R1015) to remove PCR inhibitors. ..

    Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures.
    Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and Concentrator-5 (catalog number R1015), not by ethanol precipitation. ..

    Construct:

    Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures.
    Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and Concentrator-5 (catalog number R1015), not by ethanol precipitation. ..

    Nick Translation:

    Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures.
    Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and Concentrator-5 (catalog number R1015), not by ethanol precipitation. ..

    Ethanol Precipitation:

    Article Title: Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures.
    Article Snippet: The FISH probe was constructed using an ARES AlexaFluor 647 DNA labeling kit (ThermoFisher Catalog # A21676), by nick translation. .. The DNA was constructed by PCR using dph-6 primers as described in [20] and the manufacturer’s instructions were used in all steps for nick translation, except purification was performed using Zymo RNA Clean and Concentrator-5 (catalog number R1015), not by ethanol precipitation. ..

    cDNA Synthesis:

    Article Title: Loss of host factor-mediated m 6 Am methylation of the viral RNA cap impairs SARS CoV-2 replication
    Article Snippet: The decapped RNA was ligated with an RNA adapter in a total 25 μL reaction (RNA 9.25 μL, 10x T4 RNA ligase buffer 2.50 μL, 5 μM 5′ Adapter in 3.0 μL (15 pmol total), RNasin plus 1.0 μL (40units), T4 RNA ligase 2.0 μL (NEB, M0204S), 50% PEG8000 6.25 μL, 25 mM ATP 1.0 μL) overnight at 16°C. .. The next day, RNA was purified with RNA Clean and Concentrator-5 (Zymo research, R1016) following the long RNA purification protocol and subjected to first-strand cDNA synthesis using 0.5 μg (1 μL, 50 μM stock) primer pWhSF-ORF1a-R18-655 ( ) binding to the ORF1a, and superscript RTII (ThermoFisher Scientific, Cat. 18064022). ..

    Binding Assay:

    Article Title: Loss of host factor-mediated m 6 Am methylation of the viral RNA cap impairs SARS CoV-2 replication
    Article Snippet: The decapped RNA was ligated with an RNA adapter in a total 25 μL reaction (RNA 9.25 μL, 10x T4 RNA ligase buffer 2.50 μL, 5 μM 5′ Adapter in 3.0 μL (15 pmol total), RNasin plus 1.0 μL (40units), T4 RNA ligase 2.0 μL (NEB, M0204S), 50% PEG8000 6.25 μL, 25 mM ATP 1.0 μL) overnight at 16°C. .. The next day, RNA was purified with RNA Clean and Concentrator-5 (Zymo research, R1016) following the long RNA purification protocol and subjected to first-strand cDNA synthesis using 0.5 μg (1 μL, 50 μM stock) primer pWhSF-ORF1a-R18-655 ( ) binding to the ORF1a, and superscript RTII (ThermoFisher Scientific, Cat. 18064022). ..



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    Image Search Results


    Smoke PM 2.5 summary statistics (mean and 98th percentile of all census tract estimates) by year (μg/m 3 ). Note: Acres burned were extracted from CAL FIRE Redbooks for each year ( https://www.fire.ca.gov ) and are depicted (by the Gy bars) relative to the year with the highest burned area, 2018. Total acres burned from 2008 to 2018 in order of year: 1,593,690; 451,969; 134,462; 228,599; 829,224; 601,635; 625,540; 880,899; 669,534; 1,548,429; and 1,975,086.

    Journal: GeoHealth

    Article Title: A Comparative Analysis of Wildland Fire Smoke PM 2.5 Exposure Estimates Across California From 2008 to 2018

    doi: 10.1029/2025GH001575

    Figure Lengend Snippet: Smoke PM 2.5 summary statistics (mean and 98th percentile of all census tract estimates) by year (μg/m 3 ). Note: Acres burned were extracted from CAL FIRE Redbooks for each year ( https://www.fire.ca.gov ) and are depicted (by the Gy bars) relative to the year with the highest burned area, 2018. Total acres burned from 2008 to 2018 in order of year: 1,593,690; 451,969; 134,462; 228,599; 829,224; 601,635; 625,540; 880,899; 669,534; 1,548,429; and 1,975,086.

    Article Snippet: Average smoke PM 2.5 concentrations over the 11‐year period are highest for Wilkins (Figure ), with hot spots again in northern California and throughout the Central Valley, ranging from 0.29 μg/m 3 for the least exposed tract in San Bernardino County to 10.3 μg/m 3 for the most exposed tract in Sonoma County.

    Techniques:

    Spatial metrics of intensity, frequency, and duration for smoke PM 2.5 in California, 2008–2018. Number of weeks with smoke PM 2.5 exceeding 5 μg/m 3 (top row), number of days with smoke PM 2.5 exceeding 1 μg/m 3 , indicating nonzero smoke exposure (second row), mean smoke PM 2.5 (third row), number of smoke waves (fourth row). Note: Smoke waves represent the number of instances of ≥2 consecutive days with >15 μg/m 3 wildfire PM 2.5

    Journal: GeoHealth

    Article Title: A Comparative Analysis of Wildland Fire Smoke PM 2.5 Exposure Estimates Across California From 2008 to 2018

    doi: 10.1029/2025GH001575

    Figure Lengend Snippet: Spatial metrics of intensity, frequency, and duration for smoke PM 2.5 in California, 2008–2018. Number of weeks with smoke PM 2.5 exceeding 5 μg/m 3 (top row), number of days with smoke PM 2.5 exceeding 1 μg/m 3 , indicating nonzero smoke exposure (second row), mean smoke PM 2.5 (third row), number of smoke waves (fourth row). Note: Smoke waves represent the number of instances of ≥2 consecutive days with >15 μg/m 3 wildfire PM 2.5

    Article Snippet: Average smoke PM 2.5 concentrations over the 11‐year period are highest for Wilkins (Figure ), with hot spots again in northern California and throughout the Central Valley, ranging from 0.29 μg/m 3 for the least exposed tract in San Bernardino County to 10.3 μg/m 3 for the most exposed tract in Sonoma County.

    Techniques:

    Average (red) and tract‐level (gray) smoke PM 2.5 concentrations for the first 2 weeks of each case study for each data set. Notes: Gray lines reflect individual census tract estimates, and red lines the overall average for all tracts in all counties containing any portion of the fire perimeter. Extreme observations beyond each plot's y ‐axis are not included.

    Journal: GeoHealth

    Article Title: A Comparative Analysis of Wildland Fire Smoke PM 2.5 Exposure Estimates Across California From 2008 to 2018

    doi: 10.1029/2025GH001575

    Figure Lengend Snippet: Average (red) and tract‐level (gray) smoke PM 2.5 concentrations for the first 2 weeks of each case study for each data set. Notes: Gray lines reflect individual census tract estimates, and red lines the overall average for all tracts in all counties containing any portion of the fire perimeter. Extreme observations beyond each plot's y ‐axis are not included.

    Article Snippet: Average smoke PM 2.5 concentrations over the 11‐year period are highest for Wilkins (Figure ), with hot spots again in northern California and throughout the Central Valley, ranging from 0.29 μg/m 3 for the least exposed tract in San Bernardino County to 10.3 μg/m 3 for the most exposed tract in Sonoma County.

    Techniques: